Choose a defensible model
Test variance, weighting and model order, then inspect the selected equation, fit statistics and residual diagnostics.
Free · browser-based · open source
Analytical method validation, from data to decision.
MVA turns complex statistical checks into a guided workflow for calibration, detection limits, precision, accuracy and additional validation parameters.
validation runmodel selected
Illustrative view. MVA computes results and diagnostics from the dataset you provide.
Why MVA
Rigorous validation should not be a spreadsheet maze.
Analytical validation joins experimental design, statistical testing and acceptance decisions. MVA keeps those pieces in one visible sequence.
Fragmented workflow
Calculations spread across files
- Repeated manual steps increase the opportunity for error.
- Model assumptions and acceptance criteria can be easy to overlook.
- Plots, tables and statistical checks live in separate tools.
Guided workflow
One route from input to evidence
- Automated statistical checks support a consistent protocol.
- Configurable settings adapt the analysis to the study design.
- Tables and visual diagnostics make each result easier to inspect.
The objective: reduce repetitive calculation, make the statistical reasoning explicit and give analysts more time for scientific judgement.
Workflow
From validation dataset to an interpretable result.
Each page advances the same study. Move through the core parameters, review the evidence and adjust the settings that belong to your protocol.
01
Import and inspect
Load the validation dataset and review identifiers, calibration curves and normalized analytical responses before calculation.
.txt.csv.xls.xlsx
02
Evaluate variance
Examine instrumental behaviour across concentration levels and determine whether variance is constant or concentration-dependent.
Levene testLLOQ / ULOQvariance plots
03
Select the calibration model
Compare regression strategies, weighting factors and model order; then inspect goodness of fit and residual behaviour.
OLSWLS1/x1/x²linear / quadratic
04
Determine LOD and LOQ
Calculate detection and quantification limits from the calibration data with configurable significance and calibrator settings.
Hubaux & Vosprediction intervalconfigurable α
05
Assess precision and accuracy
Calculate intra-day and inter-day CV% and bias%, then read results against acceptance criteria defined for the study.
intra-dayinter-dayCV%bias%
06
Complete the validation picture
Evaluate further parameters where they are relevant to the method and the available experimental evidence.
sensitivityselectivitycarry-overmatrix effectrecovery
Validation parameters
The calculations that turn performance into evidence.
MVA combines numerical output with tables and plots, so a result can be checked rather than simply accepted.
Work at the lower range
Estimate limits through the Hubaux and Vos approach and visualize the prediction interval behind the result.
Measure repeatability
Review intra-day and inter-day variability by concentration level through coefficients of variation.
Quantify systematic difference
Use back-calculated concentrations to evaluate intra-day and inter-day percentage bias.
Extend the assessment
Bring sensitivity, selectivity, carry-over, matrix effect and extraction recovery into the validation workspace.
Set criteria in context
Use configurable thresholds and colour-coded tables to distinguish acceptable, borderline and non-acceptable results.
Flexible by design
A protocol that fits the study.
The complete reference protocol uses nine calibration-curve replicates over three days. MVA also supports reduced designs and lets the analyst configure the structure of the available experiment.
- Adjust the number of calibration levels and replicates.
- Define curves per day and days of validation.
- Reuse the calibration evidence across multiple validation parameters.
- Keep acceptance criteria aligned with the method's intended purpose.
Important: MVA supports the workflow; the laboratory remains responsible for study design, regulatory interpretation and final fitness-for-purpose decisions.
Reference design3 days × 3 curves
days3×3curve replicates=9
The reference design strengthens reliability and enables intermediate-precision evaluation. A reduced design can be used when it is scientifically and regulatorily appropriate.
Statistical evidence
The model is selected through a visible decision path.
MVA links variance testing, regression choice, model-order comparison and residual diagnostics instead of treating them as disconnected outputs.
01
Variance
Levene's test assesses homoscedasticity across calibration levels.
02
Regression
OLS or WLS is evaluated, with 1/x and 1/x² weighting when appropriate.
03
Model order
Linear and quadratic alternatives are compared while guarding against overfitting.
04
Diagnostics
Residual tests and plots help reveal patterns the model has not explained.
Open source
Methods Validation App - source code and development history
Inspect the implementation, follow changes and contribute through the public repository.
Open GitHub → MethodsX · 2020Experimental and statistical protocol for effective validation
The published protocol underlying the coordinated evaluation of calibration and validation parameters.
Open the paper → Talanta · 2020Effective validation of chromatographic analytical methods
An applied case study demonstrating the validation strategy in analytical practice.
Open the paper →
Ready when your data is
Open the app. Keep the reasoning in view.
MVA runs in the browser, with no local installation required. Start the workflow from the application or inspect the source before you begin.
- Free access
- No installation
- Cross-platform browser access
- Open and inspectable source code
- Guided visual workflow
Support and feedback
Report a problem or help shape what comes next.
Use the public issue tracker for reproducible technical reports, or contact DataBloom to discuss a validation workflow and applied support.
GitHub issues
Describe the problem, the expected behaviour and the steps needed to reproduce it.
Open an issueTalk to DataBloom
Discuss analytical validation, custom development or integration with your laboratory workflow.
info@databloom.it
Collaboration
Academic research and applied data science.
MVA brings together analytical chemistry, statistical method development and accessible scientific software.
Methods Validation AppOpen science for analytical validation